Research interest(s)/area of expertise
- Molecular systems biology
- mRNA translation
- RNA mediated gene regulation
- Genome-wide high-throughput methods in bacteria
- Chemical biology
Currently accepting new PhD students
Sub-cellular organization of mRNA decay in bacteria
In eukaryotic cells, mRNA decay is often organized in ribonucleoprotein granules like RNA processing-bodies or stress granules. We found that bacteria can also make similar ribonucleoprotein granules that we termed bacterial ribonucleoprotein bodies (BR-bodies) composed of Ribonuclease E, protein components of the RNA degradosome, and RNA. These BR-bodies appear to be important for mRNA degradation and are assembled by liquid-liquid phase separation from the cytoplasm forming a compartment with high concentrations of the RNA degradosome and RNA. Here were's currently utilizing high-throughput mRNA decay assays and cell biology experiments to probe the role of these granules in mRNA decay.
Mechanisms of non-Shine-Dalgarno translation initiation
The mechanism of translation initiation in bacteria was first examined in E. coli, where the presence of a Shine-Dalgarno site preceding the start codon leads to the initiation of translation in the proper reading frame. Now with thousands of sequenced bacterial genomes it was discovered that less than 1/2 of all bacterial protein coding genes are preceded by a Shine-Dalgarno site. Additionally, individual bacterial species including many cyanobacteria and bacteroidetes, lack Shine-Dalgarno sites in nearly 90% of their genes! We are therefore investigating the mechanisms of non-Shine-Dalgarno initiation by utilizing Caulobacter crescentus. Caulobacter contains Shine-Dalgarno sites in only 23.5% of its genes, has a doubling time of less than 2 hours, has well established genetic tools, and has a well annotated transcriptome. We are currently utilizing ribosome profiling, translation reporters, and in vitro reconstituted translation initiation assays to dissect the factors required for non-Shine-Dalgarno initiation in Caulobacter.
- Postdoctoral Fellowship, Stanford University 2011-2015
- Ph.D. Biophysical Chemistry, Northwestern University 2011
- B.S. Microbiology, Colorado State University 2005
Awards and grants
Junior Faculty Award, WSU Academy of Scholars, 2018
NIH NIGMS MIRA Award R35GM124733, 2017-2022
Al-Husini, N., D. T. Tomares, Z. Pfaffenberger, N. S. Muthunayake, M. A. Samad, T. Zuo, O. Bitar, J. R. Aretakis, M.-H. M. Bharmal, A. Gega, J. S. Biteen, W. S. Childers and J. M. Schrader. BR-bodies provide selectively permeable condensates that stimulate mRNA decay and prevent release of decay intermediates. Molecular Cell 2020.
Aretakis, J.R., Gega, A., Schrader, J.M. Absolute measurements of mRNA translation in C. crescentus reveal important fitness costs of vitamin B12 scavenging. mSystems, 2019.
Al-Husini, N., Tomares, D.T., Bitar, O., Childers, W.S., Schrader, J.M. α-proteobacterial RNA degradosomes assemble liquid-liquid phase separated RNP bodies. Molecular Cell, 2018.
Schrader, J.M., Li, G.W., Childers, W.S., Perez, A., Weissman, J.S., Shapiro, L., McAdams, H.H. Dynamic translational regulation in Caulobacter cell cycle control. Proceedings of the National Academy of Sciences 2016
- BIO 7520 - Nucleic Acids Lab
- BIO 6510 - Molecular Interactions
- MGG 7015 - Introduction to Genetics
BIO 2200 - Introductory Microbiology
BIO 5240/7240 - Molecular Systems Biology
BIO 6994 - Technical Communication in Molecular Biotechnology
MGG 7015 - Introduction to Genetics (Led by Prof. Tiffany Cook)
BIO 7520 - Nucleic Acid Lab
BIO 6510 - Molecular Interactions
Other qualifications directly relevant to courses taught
Board Member - Michigan Branch of the American Society of Microbiology
Member of the WSU Institutional Biosafety Committee