Jared Schrader

Jared Schrader

Assistant Professor

313 577 0736

Schrader@wayne.edu

 Biological Sciences Building Room 2119

Websites

www.biochemicalphysics.com

Jared Schrader

Research Interest/Area of Expertise

  • Molecular systems biology

  • mRNA translation

  • RNA mediated gene regulation

  • Genomics/high-throughput methods in bacteria

  • Chemical biology

Research

Mechanisms of non-Shine-Dalgarno translation initiation
The mechanism of translation initiation in bacteria was first examined in E. coli, where the presence of a Shine-Dalgarno site preceding the start codon leads to the initiation of translation in the proper reading frame. Now with thousands of sequenced bacterial genomes it was discovered that less than 1/2 of all bacterial protein coding genes are preceded by a Shine-Dalgarno site. Additionally, individual bacterial species including many cyanobacteria and bacteroidetes, lack Shine-Dalgarno sites in nearly 90% of their genes! We are therefore investigating the mechanisms of non-Shine-Dalgarno initiation by utilizing Caulobacter crescentus. Caulobacter contains Shine-Dalgarno sites in only 23.5% of its genes, has a doubling time of less than 2 hours, has well established genetic tools, and has a well annotated transcriptome. We are currently utilizing ribosome profiling, translation reporters, and in vitro reconstituted translation initiation assays to dissect the factors required for non-Shine-Dalgarno initiation in Caulobacter.

Sub-cellular organization of mRNA decay in bacteria
In eukaryotic cells, mRNA decay is often organized in ribonucleoprotein granules like RNA processing-bodies or stress granules. We found that bacteria can also make similar ribonucleoprotein granules that we termed bacterial ribonucleoprotein bodies (BR-bodies) composed of Ribonuclease E, protein components of the RNA degradosome, and RNA. These BR-bodies appear to be important for mRNA degradation and are assembled by liquid-liquid phase separation from the cytoplasm forming a compartment with high concentrations of the RNA degradosome and RNA. Here were's currently utilizing high-throughput mRNA decay assays and cell biology experiments to probe the role of these granules in mRNA decay.

RNA-mediated mechanisms of cell cycle-regulation
The Caulobacter cell cycle is controlled by a genetic and biochemical circuit that functions in space and time to control the the transcription of ~20% of the entire genome. We recently found that approximately half of these mRNAs contained translational control to regulate the timing of expression. We are interested in the regulatory logic of RNA-mediated control and how it is integrated with the cell cycle-regulatory circuit.

Education – Degrees, Licenses, Certifications

  • Postdoctoral Fellowship, Stanford University 2011-2015
  • Ph.D. Biophysical Chemistry, Northwestern University 2011
  • B.S. Microbiology, Colorado State University 2005

Awards and Grants

  •  R35GM124733

Selected Publications

Schrader, J.M., Li, G.W., Childers, W.S., Perez, A., Weissman, J.S., Shapiro, L., McAdams, H.H. Dynamic translational regulation in Caulobacter cell cycle control. Proceedings of the National Academy of Sciences 2016

Schrader, J.M., Zhou, B., Li, G., Lasker, K., Childers, W.S., Williams, B., Long, T., Crosson, S., McAdams, H.H., Weissman, J.S., Shapiro, L. The coding and noncoding architecture of the Caulobacter crescentus genome. PLOS Genetics 2014
 

Currently Teaching

  • Bio 6994 - Technical Communication in Molecular Biotechnology 

Courses taught

Bio 6994 - Technical Communication in Molecular Biotechnology 

Bio 2200 - Introductory Microbiology

Bio 5060/8000 - Molecular Systems Biology

Other qualifications directly relevant to courses taught

 Board Member - Michigan Branch of the American Society of Microbiology