Takeshi Sakamoto
Associate Professor in Physics
Adjunct Professor in Department of Physiology, School of Medicine
313-577-2970
Room 287 in Physics Building
Website(s)
Department
Takeshi Sakamoto
Research interest(s)/area of expertise
Molecular motors: myosin, kinesin, & dynein; kinetics study of heart & muscle; hearng loss; migration of cancer cells
Research
His labs focus on following research areas: molecular motors, single molecule imaging, force measurement by optical trap, cell mechanics, cancer research, the simulation of ensemble works, solution kinetics.
Education
Ph.D.Awards and grants
- 2009 – 2012 K99/R00 NIH Pathway to Indipendent award
- 2015 – 2019 NSF Career Developmetal Award
Selected publications
- Gunther LK., Feng H-Z., Wei H., Raupp J., Jin J.-P., Sakamoto T*., Effect of N-terminal Extension of Cardiac Troponin I on the Ca2+ Regulation of ATP-binding and ADP Dissociation of Myosin II in Native Cardiac Myofibrils. Biochemistry, 2016, 55(12), 1887-97
- Fang J.,Liu M., Zhang X., Sakamoto T., Taatjes D.J., Jena B.P., Sun F., Woods J., Bryson T., Kowluru K., and Chen X., COPII-Dependent ER Export: A Critical Component of Insulin Biogenesis and T-Cell ER Homeostasis. Molecular Endocrinology, 2015, 29(8), 1156-69
- Bao J., Wang S., Gunther L.K., Kitairi S., Li C., and Sakamoto T.*, The Actin-bundling Protein TRIOBP-4 and -5 Promotes the Motility of Pancreatic Cancer Cells. Cancer letter, S0304-3835 (14)00433-9
- Gunther L.K., Furuta K., Boa J., Urbanowski M.K., Kojima H., Whie H.D., and Sakamoto T.*, Coupling of two non-processive myosin 5c dimers enables processive stepping along Actin filament. Scientific Reports, 2014, 9(4), 4907
- Bao J., Gunther L.K., Bielski E., Bachhawat A., Thirumurugan T, Kitajiri S., and Sakamoto T.*, R1 motif is the major actin-binding domain of TRIOBP-4, 2012, Biochemistry, 2013, 52(31), 5256-4
- Bao J., Huck D., Gunther L.K., Sellers J.R., and Sakamoto T.*, Actin structure-Dependent stepping of myosin 5a and 10 during processive movement. PLoS One, 2013, 8(9), e74936
- Nagi N.T., Saikat C., Harami G.M., Sellers J.R., Sakamoto T., and Kovacs M., A subdomain interaction at the base of the lever allosterically tunes the mechanochemical mechanism of myosin 5a. PLoS One, 2013, 8, e62640
- Martina J.A., Wu X.S., Catalfamo M., Sakamoto T., Yi C., Hammer J.A. 3rd, Imaging of Lytic granule exocytosis in CD8(+) cytotoxic T lymphocytes reveals a modified form of Full fusion. Cell Immunol., 2011, 271(2), 267-79
- Nagy N.T., Sakamoto T., Takacs B., Gyimesi M., Bikadi Z., Sellers J.R., and Kovacs M., Functional adaptation of the switch-2 nucleotide sensor enables rapid processive Translocation by myosin-5, FASEB J, 2010, 24(11), 4480-90
- Kitajiri S., Sakamoto T., Belyantseva I.A., Goodyear R.J., Stepanyan R., Fujiwara I., Bird J.E., Riazuddin S., Riazuddin S., Ahmed Z.M., Hinshaw J.E., Sellers J.R., Bartles J.R., Hammer J.A. 3rd, Richardson G.P., Griffith A.J., Forlenkov G.I., and Friedman T.B., TRIOBP is a novel Actin-bundling protein required for rootlet formation and rigidity of hail cell stereocilia. Cell, 2010, 141(5), 786-98.
- Oke O., Burgess S.A., Forgacs E., Knight P.J., Sakamoto T., Sellers J.R., White H., and Trinich J., Influence of lever structure on myosin 5a walking, Proc. Natl. Acad. Sci. U.S.A., 2010, 107, 2509-14