
Takeshi Sakamoto
Associate Professor in Physics
Adjunct Professor in Department of Physiology, School of Medicine, Wayne State University
313-577-2970
Room 287 in Physics Building
Website(s)
Takeshi Sakamoto
Research interest(s)/area of expertise
Molecular motors: Myosin, Kinesin, & Dynein; Kinetics study of Heart & Muscle; Hearng loss; Migration of Cancer cells,
Research
His labs focus on following reserach areas: molecular motors, single molecule imaging, force measurement by Optical trap, Cell mechanics, Cancer research, The simulation of ensemble works, Solution kinetics.
More information, please go to http://takeshisakamotolabs.com/
Education
Ph.D.Awards and grants
2009 ~ 2012 K99/R00 NIH Pathway to Indipendent award
2015 ~ 2019 NSF Career Developmetal Award
Selected publications
1: Gunther LK., Feng H-Z., Wei H., Raupp J., Jin J.-P., Sakamoto T*., Effect of N-terminal
Extension of Cardiac Troponin I on the Ca2+ Regulation of ATP-binding and ADP
Dissociation of Myosin II in Native Cardiac Myofibrils. Biochemistry, 2016, 55(12), 1887-
97
2: Fang J.,Liu M., Zhang X., Sakamoto T., Taatjes D.J., Jena B.P., Sun F., Woods J., Bryson
T., Kowluru K., and Chen X., COPII-Dependent ER Export: A Critical Component of
Insulin Biogenesis and -Cell ER Homeostasis. Molecular Endocrinology, 2015, 29(8),
1156-69
3: Bao J., Wang S., Gunther L.K., Kitairi S., Li C., and Sakamoto T.*, The Actin-bundling
Protein TRIOBP-4 and -5 Promotes the Motility of Pancreatic Cancer Cells.
Cancer letter, S0304-3835 (14)00433-9
4: Gunther L.K., Furuta K., Boa J., Urbanowski M.K., Kojima H., Whie H.D., and
Sakamoto T.*, Coupling of two non-processive myosin 5c dimers enables processive
stepping along Actin filament. Scientific Reports, 2014, 9(4), 4907
5: Bao J., Gunther L.K., Bielski E., Bachhawat A., Thirumurugan T, Kitajiri S., and
Sakamoto T.*, R1 motif is the major actin-binding domain of TRIOBP-4, 2012,
Biochemistry, 2013, 52(31), 5256-4
6: Bao J., Huck D., Gunther L.K., Sellers J.R., and Sakamoto T.*, Actin structure-
Dependent stepping of myosin 5a and 10 during processive movement. PLoS One, 2013,
8(9), e74936
7: Nagi N.T., Saikat C., Harami G.M., Sellers J.R., Sakamoto T., and Kovacs M.,
A subdomain interaction at the base of the lever allosterically tunes the mechanochemical
mechanism of myosin 5a. PLoS One, 2013, 8, e62640
8: Martina J.A., Wu X.S., Catalfamo M., Sakamoto T., Yi C., Hammer J.A. 3rd, Imaging of
Lytic granule exocytosis in CD8(+) cytotoxic T lymphocytes reveals a modified form of
Full fusion. Cell Immunol., 2011, 271(2), 267-79
9: Nagy N.T., Sakamoto T., Takacs B., Gyimesi M., Bikadi Z., Sellers J.R., and Kovacs M.,
Functional adaptation of the switch-2 nucleotide sensor enables rapid processive
Translocation by myosin-5, FASEB J, 2010, 24(11), 4480-90
10: Kitajiri S., Sakamoto T., Belyantseva I.A., Goodyear R.J., Stepanyan R., Fujiwara I.,
Bird J.E., Riazuddin S., Riazuddin S., Ahmed Z.M., Hinshaw J.E., Sellers J.R., Bartles
J.R., Hammer J.A. 3rd, Richardson G.P., Griffith A.J., Forlenkov G.I., and Friedman T.B.,
TRIOBP is a novel Actin-bundling protein required for rootlet formation and rigidity of
hail cell stereocilia. Cell, 2010, 141(5), 786-98.
11: Oke O., Burgess S.A., Forgacs E., Knight P.J., Sakamoto T., Sellers J.R., White H., and
Trinich J., Influence of lever structure on myosin 5a walking,
Proc. Natl. Acad. Sci. U.S.A., 2010, 107, 2509-14
Currently teaching
PHY 5340
Courses taught
General Physics (Physics I); PHY2130: W17
General Physics (Physics II): PHY2140: W13, W14, W15, W16, F19, F20
Biological Physics: PHY5750: F11, F12, F13, F14, F15
Optics PHY5340: W19, W20, W21
Thermodynamics and Statistical Physics: PHY6500: F16
Research methods in Biomedical Physics: PHY6780: W10, W11, W12