313 577-8616 (fax)
3027 Science Hall
I have had the privilege to make progressive contributions both in teaching and research, specifically in the area of nutrition, disease and metabolomics. In spite of the challenging economic environment, I have created a successful and sustainable research program, with substantial external funding, a respectable publication record and numerous presentations at local, national and international settings. This has been accomplished along with course and curriculum development,and considerable service at the departmental, university and community levels.
By educating, close mentoring, instilling enthusiasm and encouraging a team spirit, my team and I have enjoyed an active, diverse, fun and productive laboratory environment. I have successfully graduated 7 PhD, 14 MS students, together with training a host of undergraduate students. In addition, we have been able to host, train and perhaps inspire (!) high school students over the summer semesters from various communities around Metro Detroit.
Research Interest/Area of Expertise
Metabolomics in Nutrition and Disease
The interdisciplinary approach towards my research interests encompasses and connects the diversity of my academic background (MS, chemistry; PhD, Nutrition and Food Science, Postdoctoral/biotech industry experience, lipoprotein metabolism, oxidative stress and inflammation). Thus, my team investigates the effect of dietary bioactive compounds including curcumin, tocotrienols, garcinol and palm polyphenolics in the potential prevention and/or treatment of disease progression. Metabolomics is inculcated into my research matrix to scrutinize the efficacy of the bioactive compounds in the respective disease models. In addition, it serves as a sophisticated tool for the development of potential non-invasive, early risk biomarkers of disease or its progression. Non-omic methods including gene and protein expression analysis, microRNA profile modulation are also evaluated and correlated with changes in the metabolomic profiles to get a deeper mechanistic understanding.
Metabolomics: My team uses 1H NMR spectroscopy in tandem with the power of multivariate analyses, including Principal component analysis (PCA), PLSDA, OPLS. Multiple regression analysis is also used for correlation of metabolomics with genomics or other omics data. We have optimized methods for plasma (human, rodent), urine (human, rodent), cell culture extracts (human, yeast), biological tissue extract.
Disease Models Investigated : Our research has been focused on lung and pancreatic cancer and more recently on Alzheimer's Disease. Both invitro, human cancer cell lines and invivo models of disease including mice, rats, hamster models have been utilized to evaluate various aspects of the research questions.
Collaborative work includes metabolomic analysis of samples from clinical studies (Sarcodoisis lung disease, renal disease, neonatatal patients), yeast cultures and drosophylla fly extracts. In this way, I have been been able to extend my expertise to the research community.
Education – Degrees, Licenses, Certifications
- PhD, Nutrition and Food Science
- MS, Chemistry
- BS, Chemistry
Rajasinghe L., and Gupta SV (2017). Tocotrienols rich mixture inhibits cell proliferation and induces apoptosis via down-regulation of the Notch-1/NF-κB pathways in non-small cell lung cancer cells (NSCLC). J. Nutrition and Dietary Supplements. In press
Geamanu A., Bauerfeld, C., Gupta, SV, and Samavati L, (2016) Metabolomics Connect Aberrant Bioenergetic Gut microbiota and Inflammation in Sarcoidosis Patients. Metabolomics. 12(2),35 1-13 DOI 10.1007/s11306-015-0932-2.
Ji X, Usman A, Razalli NH, Sambanthamurthi R, Gupta, SV (2015). Oil palm phenolics (OPP) inhibit pancreatic cancer cell proliferation via suppression of NF-κB pathway. Anticancer Res. Jan;35(1):97-106.
Geamanu, A., Saadat, N., Goja, A., Khosla, P and Gupta, S.V. (2013) ProAlgaZyme subfraction improves the lipoprotein profile of hypercholerteolemic hamsters, while inhibiting production of betaine, carnitine and choline metabolites. J. Nutrition & Metabolism. 10:55.
Parasramka, M., Ali, S., Banerjee, S., Deryavoush, T., Sarkar, F., and Gupta, S.V. (2012) Garcinol sensitizes human pancreatic adenocarcinoma cells to gemcitabine by modulating microRNA signatures. Molecular Nutrition and Food Research. 57: 235-248 IF: 4.31
Ji,X., Wang, Z., Sarkar, F.H., and Gupta, S.V. (2012) Delta-tocotrienol augments cisplatin induced suppression of non-small cell lung cancer cells via inhibition of the Notch-1 pathway. Anticancer Research, 32(7) 2639-2645. IF:1.75
Geamanu, A., Saadat, N., Goja, A., Wadehra, M., Ji, X., and Gupta, S.V. (2012) ProAlgaZyme and its sub-fractions increase plasma HDL-cholesterol via up regulation of ApoA1, ABCA1 and SRB1 and inhibition of CETP in hypercholesterolemic hamsters. J. Nutrition and Dietary supplements. 4: 17-24. Open access: Total views: 2017 as of 11/8/2012; Total PDF downloads: 441
Parasramka, M., and Gupta, S.V. (2012) Synergistic effect of garcinol and curcumin on antiproliferative and apoptotic activity in pancreatic cancer cells. J. Oncology. Volume 2012, Article ID 709739, doi:10.1155/2012/709739
Saadat, N. and Gupta, S.V. (2012) Potential Role of Garcinol as an Anticancer Agent. J. Oncology. Volume 2012, Article ID 647206, doi:10.1155/2012/647206IF:
Ji,X., Wang, Z., Geamanu, A., Goja, A., Sarkar,, F.H., and Gupta, S.V. (2012) Delta-tocotrienol Suppresses Notch-1 Pathway by Up-regulating miR-34a in Non-small Cell Lung Cancer Cells. International J. Cancer. 131 (11)2668-677
Saadat, N., Iglayreger, H., Myers,M., Bodary,P., and Gupta, S.V. (2012) Differences in metabolomic profiles of male db/db and s/s, leptin receptor mutant mice. Physiological Genomics. 44(6):374-81.
Ji, X., Wang, Z., Geamanu, A., Sarkar, F.H., Gupta, S.V. (2011) Inhibition of cell growth and induction of apoptosis in non-small cell lung cancer cells by delta-tocotrienol is associated with Notch-1 down-regulation. J. Cell. Biochemistry. 112(10): 2773-83.
Parasramka, M., and Gupta, S.V. (2011) Garcinol Inhibits Cell Proliferation and Promotes Apoptosis in Pancreatic Adenocarcinoma Cells. J. Nutrition and Cancer. 63(3):456-65.
- NFS 7000 Nutritional Metabolomics and Bioinformatics
- NFS 5130 Food Chemistry
- NFS 2130 Food Science
- CB 7460 Mechanisms in Neoplasia. Alterations in cell signaling (team taught)
Other qualifications directly relevant to courses taught
Licensed to teach the SIMCA multivariate analsys software program. This is used to perform data analysis exercises in the metabolomics class (NFS 7000)