Robert Thomas

Robert Thomas

Senior Lecturer

313-577-3548

313-577-6891 (fax)

robert.a.thomas@wayne.edu

Department of Biological Sciences
Wayne State University
Biological Sciences Building, Rm 2121
5047 Gullen Mall
Detroit, MI 48202

Curriculum Vitae

Robert Thomas

Department

Biological Sciences

Education – Degrees, Licenses, Certifications

  • York College of CUNY B.Sc. Biology, Jamaica, NY 06/80
  • Wayne State University M. Sc. Bacteriology 05/83
  • Wayne State University Ph.D. Molecular Virology 05/92
  • Postdoctoral Fellowship, Wayne State University, School of Medicine, Center for Molecular Medicine and Genetics, Detroit, MI. 1993-1995.

Selected Publications

1. Bergh, S.T., Koziel, M.G., Huang, S.C., Thomas, R.A., Gilley, D.P. Siegel, A. (1985). The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain). Nucleic Acids Res. 13 (23), 8507-8518
2. Lewis LA, Li K, Bharosay M, Cannella M, Jorgenson V, Thomas R, Pena D, Velez M, Pereira B, Sassine A. (1990). Characterization of gentamicin-resistant respiratory-deficient (res-) variant strains of Staphylococcus aureus. Microbiol Immunol 1990;34(7):587-605
3. Thomas R.A., Pena, D, Siegel, A.S. (1993). Pepper ringspot virus RNA 1, complete sequence. 6828 bp ss-RNA linear. Genebank submission NC_003669. gi:20178598
4. Zachar V, Thomas RA, Goustin AS. (1993). Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples. Nucleic Acids Res Apr 25;21(8):2017-8
5. Zachar, V., Thomas, R. A., Jones, T. and Goustin A. S. (1994). Vertical transmission c- HIV- detection of proviral DNA in placental trophoblast. AIDS 8, 129-130.
6. Zachar, V., Ebbesen, P., Thomas, R. A., Zacharaova, V. and Goustin, A. S. (1994). Basal and tat-transactivated expression from the human-immunodeficiency-virus type-l long terminal repeat in human placental trophoblast rules out promoter-enhancer activation as the partial block to viral replication. Journal of General Virology. 75, 1461-1468.
7. Zachar, V., Zacharova, V., Fink, T., Thomas, R.A., King, B.R., Ebbesen, P., Jones, T.B. and Goustin, A.S. (1999). Genetic Analysis Reveals Ongoing HIV Type 1 Evolution in Infected Human Placental Trophoblast. AIDS Research and Human Retroviruses 15, Number 18, pp. 1673-1683.
8. Hyun, T.S., Cotter II, M.A., Thomas R.A. and Robertson, E.S. (2001). The Latency-Associated Nuclear Antigen Encoded by Kaposi’s Sarcoma-Associated Herpesvirus Interacts with Tat and Activates the Long Terminal Repeat of Human Immunodeficiency Virus Type I. Journal of Virology. 75(18):8761-71.
9. Abdel-Haq, N.M., R. A. Thomas, et al. (2003). Increased prevalence of G1P[4] genotype among children with rotavirus-associated gastroenteritis in metropolitan Detroit. J Clin Microbiol 41(6):2680-2.
10. Kang, J., Chen, C., Smolinski, J., Thomas, R., Tucker, J., and Auner, G., (2006) Simulation and optimization of a flow-through micro PCR chip, in NSTI Nanotech, Boston, MA, pp. 585-588.
11. Banda, M., Bommineni, A., Thomas, R.A. Luckinbill, L, Tucker, J.D. (2008) Evaluation and validation of housekeeping genes in response to ionizing radiation and chemical exposure for normalizing RNA expression in real time PCR. Mutation Research 649:126-134.
12. Kulkarni, R., Reither, A., Thomas, R.A., Tucker J.D., (2009) Mitochondrial mutant cells are hypersensitive to ionizing radiation, phleomycin and mitomycin C. Mutation Research – Fundamental and Molecular Mechanisms of Mutagenesis, accepted for publication 1/23/09.
13. Asur, R.S., Thomas, R.A., and Tucker, J.D. (2009) Chemical induction of the bystander effect in normal human lymphoblastoid cells. Mutation Research – Genetic Toxicology and Environmental Mutagenesis, accepted for publication on 2/25/09.
14. Tucker JD, Suter W, Petibone DM, Thomas RA, Bailey NL, Zhou Y, Zhao Y, Muniz R, Kumar V. (2009) Cytogenetic assessment of methylphenidate treatment in pediatric patients treated for attention deficit hyperactivity disorder. Mutat Res. 2009 Jun-Jul;677(1-2):53-8. Epub 2009 May 22. PubMed PMID: 19465145.
15. Kulkarni R, Thomas RA, Tucker JD. (2010) Expression of DNA repair and apoptosis genes in mitochondrial mutant and normal cells following exposure to ionizing radiation. 2010 Environ Mol Mutagen. 2010 Aug 25. [Epub ahead of print] PubMed PMID: 20740641.
16. Kulkarni R, Marples B, Balasubramaniam M, Thomas RA, Tucker JD. (2010) Mitochondrial gene expression changes in normal and mitochondrial mutant cells after exposure to ionizing radiation. Radiat Res. 2010 May;173(5):635-44. PubMed PMID: 20426663.
17. Asur R, Balasubramaniam M, Marples B, Thomas RA, Tucker JD. (2010) Bystander effects induced by chemicals and ionizing radiation: evaluation of changes in gene expression of downstream MAPK targets. Mutagenesis. 2010 May;25(3):271-9. Epub 2010 Feb 3. PubMed PMID: 20130020.
18. Asur R, Balasubramaniam M, Marples B, Thomas RA, Tucker JD. (2010) Involvement of MAPK proteins in bystander effects induced by chemicals and ionizing radiation. Mutat Res. 2010 Apr 1;686(1-2):15-29. Epub 2009 Dec 23. PubMed PMID: 20034502.
19. Hughes-Chinkhota CN, Banda M; Smolinski JM, Thomas RA, Petibone DM, Tucker JD, Auner GW. (2011) Oligonucleotide immobilization using 10-(carbomethoxy)decyl-dimethylchlorosilane for mRNA isolation and cDNA synthesis on a microfluidic chip. Sensors and Actuators B: Chemical, Volume 155, Issue 2, 20 July 2011, Pages 437-445.
20. Joiner MC, Thomas RA, Grever WE, Smolinski JM, Divine GW, Konski AA, Auner GW, Tucker JD. (2011) Developing point of care and high-throughput biological assays for determining absorbed radiation dose. Radiotherapy and Oncology (Accepted for publication May 6, 2011).
21. Rajendran, S., Harrison, S.H., Thomas, R.A. and Tucker, J.D. (2011) The role of mitochondria in the radiation-induced bystander effect in human lymphoblastoid cells. Radiation Research 175:159-171.
22. Kulkarni, R, Thomas, RA, Tucker, JD. (2011) Expression of DNA Repair and Apoptosis Genes in Mitochondrial Mutant and Normal Cells Following Exposure to Ionizing Radiation. Environ Mol Mutagen. Apr;52(3):229-37.
23. Tucker JD, Grever WE, Joiner MC, Konski AA, Thomas RA, Smolinski JM, Divine GW and Auner GW. (2012) Gene Expression-Based Detection of Radiation Exposure in Mice after Treatment with Granulocyte Colony-Stimulating Factor and Lipopolysaccharide. Radiat Res. 177, 209–219
24. Tucker, JD, Divine, GW, Grever, WE, Thomas, RA, Joiner, MC, Smolinski, JM, Auner, GW. (2013) Gene Expression-Based Dosimetry by Dose and Time in Mice Following Acute Radiation Exposure. Plos One. Dec 16;8(12):E83390.
25. Tucker, JD, Joiner, MC, Thomas, RA, Grever, WE, Bakhmutsky, MV, Chinkhota, CN, Smolinski, JM, Divine, GW, Auner, GW. (2014) Accurate Gene Expression-based Biodosimetry Using a Minimal Set of Human Gene Transcripts. Int J Radiat Oncol Biol Phys. Mar 15;88(4):933-9
26. T. J. Hadden, C. Chong, R.A. Thomas, A. Sodja. (2017) Spatial And Developmental Expression Profile of Aaeg-Obp10, An Odorant-Binding Protein Gene, In Olfactory And Non-Olfactory Tissues of The Mosquito, Aedes aegypti. Insect Biochemistry and Molecular Biology. (submitted)
 

Courses taught

BIO 1030 (LS) Biology Today, 3 credits, Winter 2018
BIO 1030 (LS) Biology Today (Honors), 3 credits, Winter 2018
BIO 1510 (LS) Basic Life Mechanisms (Engineers), 3 credit, Winter 2018
BIO 1510 (LS) Basic Life Mechanisms (Non Engineers), 4 credit, Winter 2018

BIO 1510 (2 sections)(LS) Basic Life Mechanisms (Engineers), 3 credit, Fall 2018
BIO 1510 (2 sections)(LS) Basic Life Mechanisms (Non Engineers), 4 credit, Fall 2018
BIO 1510 (LS) Basic Life Mechanisms (Honors), 4 credit, Fall 2018